ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2013, Vol. 44 ›› Issue (9): 1462-1467.doi: 10.11843/j.issn.0366-6964.2013.09.018

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Effect of Additional Boron on Tibias Osteogenesis of African Ostrich Chicks

ZHU Dai-yun1, WANG Lei1, CHEN Min1, 2, ZHENG Xin-ting1, PENG Ke-mei1*   

  1. (1. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; 2. Xinyang Agricultural College, Xinyang 464000, China
  • Received:2013-03-15 Online:2013-09-23 Published:2013-09-23

Abstract:

The aim of the present study was to find out the effects of drinking boron on osteogenesis of tibias of ostrich chicks. Sixteen ostrich chicks were randomly assigned to four groups. The ostrich chicks were fed with 0 (group A), 100 (group B), 200 (group C), and 400 mg·L-1(group D) of additional boron in water for 30 d. At the end of experiment time, the ostrich chicks were slaughtered and their top quarter of tibias were obtained, then the samples were made into paraffin sections, observed and photographed under Olympus DHP microphotograph. The results showed as follows: 1) The width, the density of trabeculae area and the marrow cavity increased significantly in others three groups, especially in group C, compared with the Group A; 2) Perimeter, area, and relative volume of trabeculae increased significantly and the maximum were occurred in group C. There were a very large number of osteoblasts and osteoclasts on the surface of trabeculae in group C. The trabecular separating degree in Group C was the minimum and significantly lower than in Group A and Group B, but had no significant difference compared with Group D; 3) In Group D, chondrocytes became focally vacuolated gradually in calcified cartilage matrix area, nuclear appeared pyknotic phenomenon, finally degradation and death appeared. These results indicated that the additional boron supplemented in drinking could promote obviously tibias osteogenesis of African Ostrich Chicks, and 200 mg·L-1 supplement boron in the drinking water appeared to be the most beneficial.

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